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SLS was measured using a Zetasizer Nano-ZS instrument (Malvern, Worcestershire, UK) at 25 °C following established protocols [49]. The molecular weight of protein in solution was determined by static light scattering (SLS) from the Debye plot of (KC/Rθ) vs. protein concentration [49], which ranged from 0.05 to 1.2 mg/mL. Peptide samples were filtered through a 0.02 µm Whatman Anotop® 10 syringe filter (GE Healthcare, Freiburg, Germany) before the measurements. The Rayleigh ratio was calibrated using toluene, as instructed by the manufacturer.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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