2.4. Fluconazole Susceptibility Testing

Of the 251 C. neoformans isolates, antifungal susceptibility testing was performed for 105 isolates: 60 randomly-selected VNI (from a total of 206), 25 VNII, 15 VNB, and five VNIV. We determined fluconazole minimum inhibitory concentration (MIC) values (range: 0.125 µg/mL to 64 µg/mL) using custom-made broth microdilution panels (NICD, Johannesburg) prepared, inoculated, and read according to susceptibility testing methods published previously and Clinical and Laboratory Standards Institute M27-A3 and M60 recommendations [21,22,23]. We interpreted fluconazole MIC values using published epidemiological cutoff values (ECVs) [24].