2.5. Dry Biomass Concentration

The fungal biomass was estimated as dry weight by separating the fermentation broth and mycelia using 48 µm nylon mesh filter (SEFAR, Nitex, Heiden, Switzerland). The filters containing the collected biomass were dried in a hot air oven (SNB 100, Memmert, Schwabach, Germany) at 105 °C for 17 h. The dried filters were left to cool in a desiccator for 30 m until they reached the room temperature, and were then weighed. The concentration of dry biomass in the culture broth, denominated as DBW, is calculated from the following equation (Equation (3)):

where, W₂ is the weight of the filter with biomass obtained after drying (g), W₁ is the weight of the corresponding empty filter (g), and V is the volume of sample (L).