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2.12. Super Oxide Dismutase (SOD) Activity Assay
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The SOD activity was determined using a SOD Assay Kit-WST (Sigma-Aldrich, St. Louis, MO, USA). Protein lysates were prepared as described by the manufacturer’s instructions. Briefly, the protein lysates were mixed with WST and enzyme working solution and incubated for 20 min at 37 °C. After incubation, the amount of water-soluble formazan dye was measured according to the absorbance at 450 nm using a microplate reader.
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