Modified neurological severity score (mNSS)

With reference to a previous study (33), scoring was performed using an 18-point mNSS system to evaluate the neurological function of the mice, where 0 is a normal score, and 18 is the maximal deficit score. The mNSS assessment includes four neurological tests, namely, a fine motor function measurement scale, sensory ability test, beam balance test, and absence of reflection and abnormal motion. Data were analyzed using a Kruskal-Wallis test followed by Steel-Dwass tests to compare between multiple groups.