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2.5. TaqMan Quantitative Methylation-Specific PCR

YW Yu-Han Wang
SC Shih-Ching Chang
MA Muhamad Ansar
CH Chin-Sheng Hung
RL Ruo-Kai Lin
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The EpiTect Fast DNA Bisulfite Conversion Kit (Qiagen; Cat. No. 59826) was used in the bisulfite conversion of genomic DNA. The level of DNA methylation was measured using a TaqMan quantitative methylation-specific PCR (QMSP). Our lab performed QMSP with specific primers and a methyl-TaqMan probe using the SensiFASTTM Probe No-ROX Kit (Bioline, London, UK; Cat. No. BIO-86020). The reference gene was beta-actin (ACTB). Table S3 presents the primers.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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