Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
RWPE-2 cells were seeded into a 96-well plate following transfection with miRNA-1297 inhibitor for 24 or 48 h. Next, 200 µl medium was added to each well, with 10 µl freshly prepared MTT (5 mg/ml) and 100 µl dissolved formazan liquid (dissolved in PBS). Subsequently, the optical density (OD) at 490 nm of each well was measured. The ratio of viable cells was calculated by dividing OD readings of the transfected groups into the control group.
Copyright and License information: ©2021 Wang et al.
This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
