The wild-type and mutant mRNA sequences encoding ENAM tagged with EGFP were submitted to the RNAfold WebServer (http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/RNAfold.cgi (accessed on 16 February 2021)). Translation in all three frames was analyzed using the Expasy translate tool (https://web.expasy.org/translate/ (accessed on 16 February 2021)) to detect upstream open reading frames (uORFs) and upstream start codons (uAUGs).
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