In the morning (8.00–10.00 a.m.), and after an overnight fast of at least 8 h, the blood samples were collected and subsequently stored at −80 °C until processed.
Serum high-sensitivity (hs) CRP levels were analyzed by CardioPhase® (Siemens Healthcare Diagnostics, Marburg, Germany), based on particle-enhanced immunonephelometry. The coefficient of variation (CV) of intra- and interassay was <7%.
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