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To measure Bax or PUMA promoter activity, H1299 cells were transfected with expression vectors encoding p53, PARP1 and HIPK2, along with the PUMA- or Bax-luciferase reporter vector (kindly provided by Dr G D'Orazi, Regina Elena Cancer Institute, Rome, Italy) and control pRL-TK plasmids. At 24 h after the transfection, dual luciferase activity assay was carried out using dual luciferase reporter assay kit (Promega) according to the manufacturer's protocol.
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Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
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