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Nestlet shredding test

AO Amanda R. de Oliveira
AR Adriano E. Reimer
GS Gregory J. Simandl
SN Sumedh S. Nagrale
AW Alik S. Widge
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The experimental protocol for the nestlet shredding test was based on that described by Angoa-Pérez et al.39. The apparatus used in this experiment consisted of a polypropylene cage with the floor filled ~5 cm deep with bedding, and containing a cotton square of known weight that was placed on top of the bedding. The animal was placed individually in the center of the cage and left for 15 min. At the end of the test, the rat was removed, and the largest remaining intact portion of the cotton square was removed and allowed to dry. After 48 h, this portion of the cotton was weighed to calculate the percentage of nestlet shredded.

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