To evaluate dynamic histomorphometry, mice of 8–10 weeks and 25 weeks of age were injected with calcein 10 mg/kg (Sigma) seven days and alizarin complexone 30 mg/kg (Sigma) intraperitoneally two days before they were sacrificed. Seven-micrometer sections of femora were analyzed under a fluorescent microscope (Leica DMR). Blinded researcher marked bone tissue, single, and double labels in Osteomeasure software (OsteoMetrics, Decatur, USA) (https://www.osteometrics.com). The software then automatically calculated the mineral apposition rate (MAR) and bone formation rate (BFR).
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