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Filipin III cholesterol staining
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H1299 cells grown on coverslips were fixed with 4% PFA at RT for 10 min and then stained with 5 μg/ml filipin-III (Cayman, USA) in PBS/1% FBS solution at RT for 2 h in the dark. The stained cells were examined under an LSM700 confocal microscope (Carl Zeiss, Germany).
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