The axolotl transcriptome was obtained from https://axolotl-omics.org and annotated using two approaches: (i) the nucleotides were BLASTed (Altschul et al., 1990) against the UniProt database (BLASTx, e-value threshold 1e-5) and (ii) amino acid sequences were obtained based on the predicted open reading frames (ORFs) by TransDecoder and then BLASTed against the UniProt database (BLASTp, e-value threshold 1-e5). Various organism protein names were mapped to human gene symbols via the R package biomaRt (Durinck et al., 2005).
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