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The Tumour Necrosis Factor alpha (TNF-α) inhibitory assay of the S. mangifera fractions was performed using an ELISA kit (Thermo Scientific, Waltham, USA) for quantification of TNF-α and IL-6 in the experimental rats’ serum. In accordance with the instruction leaflet, absorbance was measured at 450 and 550 nm on an ELISA plate reader.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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