4.6. LNCaP Cell Culture

The LNCaP human cell line was obtained from the Banco de Células do Rio de Janeiro (code 0149) (Rio de Janeiro, RJ, Brazil). LNCaP cells (passage 3–6) were grown in RPMI-1640 medium (Sigma-Aldrich—Darmstadt, Germany) and supplemented with 10% (v/v) fetal bovine serum (Sigma-Aldrich—Darmstadt, Germany) and 1% (v/v) penicillin and streptomycin antibiotic solution (Sigma-Aldrich—Darmstadt, Germany). The cells were kept in humidified air containing 5% CO₂ at 37 °C. The cells were grown to confluence and then harvested via trypsinization. After centrifugation (1200 rpm, 5 min), the cells were resuspended in RPMI-1640 medium for in vitro binding and internalization assays or in a 1:1 mixture of Matrigel and RPMI-1640 medium for development of the prostate tumor animal model.