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To validate the expression of human wild-type α-synuclein in the basal ganglia, the rats were tested for motor asymmetry 10 weeks after surgery. The animals were recorded with a video camera for 5 min, while they were freely moving in a Plexiglas cylinder. Paw touches on the side of the cylinder were counted offline and blinded to the group. The ratio of touches of contralateral paw to total touches was computed. Animals with an expression of human α-synuclein were hypothesized to use the contralateral paw less than the control animals.
Copyright and License information: ©2021 Østergaard et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
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