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SS Souvarish Sarkar
FB Farah Bardai
AO Abby L. Olsen
KL Kelly M. Lohr
YZ Ying-Yi Zhang
MF Mel B. Feany
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All reported n values are biological replicates. The sample sizes used were similar to the ones reported in previous publications [35, 36]. For Drosophila immunostaining, genetic reporter and dye labeling experiments the sample size was 6 per genotype and time point. Western blot quantifications were performed on at least three independent blots. The sample sizes for mouse experiments were determined by power analyses. For the histological studies we determined that a sample size of 5 is sufficient to detect 20 % difference between control and experimental genotypes with a power of 80 %. Exact sample size for each experiment is provided in the figure legends. Data collection and analysis in mouse experiments were performed blinded to the conditions of the experiment. Data analysis on Drosophila sections was also performed blinded. Statistical analysis was performed using one-way ANOVA and multiple comparisons among the datasets were performed. Variance was similar between groups compared.

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