BrdU Cell Proliferation Assay

JF Jingjing Fa
XZ Xiaoqing Zhang
XZ Xiaoping Zhang
MQ Ming Qi
XZ Xingyu Zhang
QF Qihua Fu
ZX Zhuoming Xu
YG Yunqian Gao
BW Bo Wang
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The cell proliferation assay was performed using TransDetect EdU Flow Cytometry Kit-647 Fluorophore (TransGen Biotech, Beijing, China). Cells were cultured with EdU solution for 2 h and fixed by 1× EdU permeabilization buffer. The assay was visualized by the FACScan system (Bio-Rad Laboratories, Inc., Hercules, CA, United States).

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