Culture of the Human Ovarian Cancer Cell Line (SKOV3)

SKOV3 was purchased from the European Collection of Authenticated Cell Cultures (ECACC, Wiltshire, England). The SKOV3 cells were cultured in the basal Rosewell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% fetal bovine serum (FBS), 2 mM GlutaMAX, and 1% antibiotics (penicillin-streptomycin). The frozen vials containing SKOV3 cells were rapidly thawed in 37°C water bath using the standard thawing procedure. The cells were cultured in a humidified 5% CO₂ incubator at 37°C.