2.11. Oil Red O (ORO), hematoxylin and eosin (H&E), and Picrosirius red staining and immunostaining

Yanyong Xu; Yingdong Zhu; Shuwei Hu; Xiaoli Pan; Fathima Cassim Bawa; Helen H. Wang; David Q.-H. Wang; Liya Yin; Yanqiao Zhang

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2.11. Oil Red O (ORO), hematoxylin and eosin (H&E), and Picrosirius red staining and immunostaining

YX Yanyong Xu

YZ Yingdong Zhu

SH Shuwei Hu

XP Xiaoli Pan

FB Fathima Cassim Bawa

HW Helen H. Wang

DW David Q.-H. Wang

LY Liya Yin

YZ Yanqiao Zhang

This method is extracted from research article: Mol Metab, Apr 2021

Hepatocyte miR-34a is a key regulator in the development and progression of non-alcoholic fatty liver disease

DOI: 10.1016/j.molmet.2021.101244

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Liver tissues were fixed in 10% formalin and then embedded in OCT or paraffin. Liver sections were stained with ORO, H&E, or Picrosirius red. Liver sections were also immunostained using an F4/80 antibody (Abcam, cat. #ab6640) and an ABC-HRP kit from Vector Laboratories (cat. #PK-4001). Images were acquired using an Olympus microscope.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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