PRA/RPLP was pulverized to particle size below 0.85 mm. 5 g of the particles was extracted by 25 mL of 70% (v/v) ethanol with ultrasonic extraction method for 40 min. The extract was let to stand at room temperature and 70% (v/v) ethanol was added to make up the weight loss during the extraction. The extract was centrifuged at 16000 rpm for 5 min, and the supernatant was used for analysis.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.