LysoTracker is an acidotropic dye that stains cellular acidic compartments, including lysosomes and autolysosomes. It has been used to detect autophagy-associated lysosomal activity from yeast to human [58, 59]. LysoTracker Red staining has proven to be an effective indicator of autophagy in B. mori fat body [10]. Newly collected fat body tissues were fragmented by forceps, washed with PBS, stained with LysoTracker Red DND-99 at a final concentration of 50 nM (L7528, Thermo Fisher Scientific, USA) for 5 min at 37℃, and washed again with PBS 3 times. The LysoTracker Red staining of the samples was observed under a Nikon Eclipse C1 confocal microscope.
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