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Antibodies

LT Luke W. Thomas
CE Cinzia Esposito
RM Rachel E. Morgan
SP Stacey Price
JY Jamie Young
SW Steven P. Williams
LM Lucas A. Maddalena
UM Ultan McDermott
MA Margaret Ashcroft
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Antibodies used were as follows, with dilutions in parentheses: rabbit polyclonal NDUFB10 (ab196019, 1:2000) from Abcam; rabbit polyclonal SDHA (11998, 1:1000) from Cell Signaling Technology; rabbit polyclonal UQCRC2 (ab14745, 1:1000) from Abcam; rabbit polyclonal COXIV (4850, 1:10,000) from Cell Signaling Technology; rabbit polyclonal BNIP3 (HPA003015, 1:2000) from Cambridge Biosciences; rabbit polyclonal SDHC (PA5-79966, 1:1000) from Invitrogen; mouse monoclonal β-Actin (ab6276, 1:10,000) antibody from Abcam; donkey anti-rabbit (NA934, 1:1000) and anti-mouse (NA931, 1:1000) horseradish peroxidase (HRP)-linked secondary antibodies from VWR. Western blot signal intensity was measured per lane using ImageJ (NIH) analysis software. Sample protein band intensities were normalised to the load control protein β-Actin. Relative band intensities were calculated relative to the internal control sample.

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