Measurement of cell culture media glucose and lactate

DW Dong Wang
RH Robert Hartman
CH Chao Han
CZ Chao-ming Zhou
BC Brandon Couch
MM Matias Malkamaki
VR Vera Roginskaya
BH Bennett Van Houten
SM Steven J. Mullett
SW Stacy G. Wendell
MJ Michael J. Jurczak
JK James Kang
JL Joon Lee
GS Gwendolyn Sowa
NV Nam Vo
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P1 rabbit NP and AF cells were grown on monolayer cultures in Gibco Hams F12 containing 23mM glucose and 10% FBS at 5% O2. Glucose and lactate concentrations in the cell culture media were quantified at different time points (2, 6, 12, 24, 48, 72h) using Accutrend Plus System (Roche Diagnostics, Switzerland) for glucose (used 10l media/time point) and lactate (0.7l media/time point) [20].

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