MeRIP-qPCR

Methylated RNA immunoprecipitation (MeRIP) is widely used in m⁶A related studies. Immunoprecipitated and purified with m⁶A antibody, m⁶A marked RNAs could be subjected to reverse transcription and qPCR to confirm the m⁶A enrichment of RNA of interest (ROI) or to compare m⁶A enrichment of ROI between different samples/treatments [7] (Fig. 3A).

Experiments identify m⁶A specific regulation of individual RNA. Shown here is schematic diagrams of (A) methylated RNA immunoprecipitation (MeRIP)-qPCR, RNA immunoprecipitation (RIP)-qPCR, cross-linking immunoprecipitation (CLIP)-qPCR, (B) dual luciferase reporter assay, (C) site-specific cleavage, and radioactive-labeling followed by ligation-assisted extraction and thin-layer chromatography (SCARLET) technologies. m⁶A, N⁶-methyladenosine; IP, immunoprecipitation; qPCR, quantitative polymerase chain reaction; LUC, luciferase; CMV, cytomegalovirus promoter; A, adenosine; TLC, thin-layer chromatography.