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Vero cells were seeded into a 12-well plate. The medium containing recombinant ZIKV was 1:10 dilute in DMEM containing 2% FBS and then added to Vero cells. After 2 h incubation, remove the media and add 1 ml 1% low-melting agarose. After another 4 days, cells were fixed with 4% formaldehyde for 1 h and stained by 1% crystal violet.
Copyright and License information: ©2021 Ci, Yang, Xu, Qin and Shi.
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