The rats' stomachs in the different groups were collected, opened, and cleaned with saline and preserved in 10 % neutral buffered formaldehyde for 24 h and processed for histological assessment. The histological changes were studied in hematoxylin and eosin-stained sections by a qualified specialist, unaware of the different groups under a light microscope.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.