LC–MS/MS analysis of interacting proteins

FG Feng Gao
XZ Xiaojun Zhang
SW Shuyue Wang
LZ Lihua Zheng
YS Ying Sun
GW Guannan Wang
ZS Zhenbo Song
YB Yongli Bao
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The total protein of Huh7 cells was separated by polyacrylamide gel electrophoresis (SDS-PAGE) and the protein bands (50 kDa) were excised after Coomassie brilliant blue staining. After decolorization, dehydration, protein reduction, protein alkylation, enzymolysis and extraction, the processed peptides were analyzed using a liquid chromatography masss pectrometry/mass spectrometry (LC–MS/MS) method to obtain the proteins specifically binding to TSP50 in Huh7 cells and characterization of the peptides was carried out by Sangon Biotech.

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