Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Measurement of Nitric Oxide Production and Cell Viability
Ask a
question
Favorite
Cell culture medium was harvested after treatment with or without LPS (100 ng/ml) in the presence or absence of DMF. NO production was analyzed by the colorimetric Griess assay to determine the concentration of nitrite, a stable NO metabolite. The absorbance was detected at 540 nm by a microplate reader. Cell viability was determined by the MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyl tetrazolium bromide] assay, and the sample absorbance was detected at 570 nm.
Copyright and License information: ©2021 Lee, Gupta, Park, Yang and Song.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
