Apomorphine-Induced Rotation Test

Apomorphine-induced rotation test was performed at 6 weeks after unilateral 6-OHDA lesions to assess the variability of the unilateral 6-OHDA-induced PD rats. Rats were assigned separately into a square cage. A dopamine receptor agonist, apomorphine (0.5 mg/kgBW in 0.9% NaCl and 1% ascorbic acid) (Sigma-Aldrich, Darmstadt, Germany), was injected subcutaneously to trigger contralateral rotation. Contralateral rotations were recorded for 1 hour. Contralateral rotations induced by apomorphine reflect the dopaminergic neuron loss. The test was repeated at 4 and 8 weeks after supplementations to assess the supplementations effects.