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To assess protein content of the samples, the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) was used. For this 25 μl of each sample and 200 μl of reagents mix were pipetted in a 96-well plate. Reactions were incubated at 37 °C for 30 min and the linear absorbance at 562 nm was read with a photometer. Imidazole has a non-negligible absorbance at 560 nm and can thus create artefacts in the assay. For this reason, samples which contained imidazole from the elution buffer used in the IMAC protocol were washed in an ultrafiltration unit prior to measurement.
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