Cytokine Release Measurement by ELISA

BR Benoit T. Roux
JH James A. Heward
LD Louise E. Donnelly
SJ Simon W. Jones
ML Mark A. Lindsay
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Following stimulation of A549, THP-1, and RAW 264.7 cells, supernatants were collected and measurements of human and mouse IL6 were made by ELISA (R&D Systems) according to the manufacturer’s guidelines. Results are expressed in percentage of maximum response of control stimulation.

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