To evaluate the behavior of three groups of mice in the open field, mice were individually placed into a 40*40 cm center area of a brightly illuminated open field arena. The bottom was divided into a 4*4 grid, 16 equal sized (10 cm*10 cm) squares. Mice were allowed to explore the stage undisturbed for 10 min. Video tracking software was used to count the time the mice entered the central bright area (the middle four squares). And the excretion rate of the three groups of mice during the test was counted.
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