Reproducibility of data

For most figures, labels from representative cases are presented. However, injections in all BLA nuclei were repeated. The following are sample sizes for anterograde tracer injections: BLA.am (n = 8), BLA.al (n = 6), BLA.ac (n = 7), BLAv (n = 2), BLAp (n = 2), BMAp (n = 2), and LA (n = 2). For retrograde tracer injections, repeated sets of injections were made for each BLA nucleus. The consistency of labeling across repeated cases is clear from manual analysis of the data (Supplementary Fig. ^4b). The label similarity also is evidenced in the output of the 2D hierarchical clustering analysis (described below), which groups injection sites based on the commonality of their projection targets. As can be seen in Supplementary Fig. ^6a, BLA.am injections grouped together, while BLA.al and BLA.ac injections formed their individual groups. This was the case despite the different anterograde tracers used across the experiments (PHAL or AAV). As additional confirmation of reproducibility, BLA.am, BLA.al, and BLA.ac anterograde injections were repeated in female mice. Supplementary Fig. ^15a–c shows the similarity in brain-wide label patterns from the same BLAa domain in males and females.