A wire hang test57 was employed to assess whole-body muscle strength and endurance. Mice were suspended by the forelimbs at the centre of a metallic wire (1 mm in diameter, 65 cm in length), which is elevated at 40 cm above the surface level. As the primary outcome, the holding impulse (g*s) is calculated as the product of the body mass (g) and the longest suspension time (s) during the 3-min test period. These studies were blinded to testers and the mice being analysed were randomized.
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