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All plasmids used in this study are listed in Table 1. Plasmids were made by conventional restriction enzyme based cloning or by use of the Gateway recombination system (ThermoFisher). Gateway LR reactions were performed as described in the instruction manual. Point mutations and deletion were carried out using the Site-directed-mutagenesis kit from STRATAGENE, using the primers described in Table 2. The oligonucleotides were ordered from ThermoFisher. All plasmids were verified by DNA sequencing (BigDye, Applied Biosystems, 4337455).
Copyright and License information: ©2021 Garcia-Garcia et al ©2021 Michael MassiahThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2021 Michael MassiahThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ©2021 Michael MassiahThis is an open access article distributed under the terms of the , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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