2-AB labelling of N-glycans

AR Ana Lúcia Rebelo
FG Francesco Gubinelli
PR Pauline Roost
CJ Caroline Jan
EB Emmanuel Brouillet
NC Nadja Van Camp
RD Richard R. Drake
RS Radka Saldova
AP Abhay Pandit
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Released N-glycans were fluorescently labelled with 2-aminobenzamide (2-AB) by reductive amination [43, 44]. Briefly, the 2-AB labelling solution containing sodium cyanoborohydride was added to the glycans and incubated at 65 °C for 2 h. The excess 2-AB solution was removed by absorption on Whatman 3MM paper (Merck, Ireland) in acetonitrile washes, being the clean labelled glycans eluted in water [45].

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