Released N-glycans were fluorescently labelled with 2-aminobenzamide (2-AB) by reductive amination [43, 44]. Briefly, the 2-AB labelling solution containing sodium cyanoborohydride was added to the glycans and incubated at 65 °C for 2 h. The excess 2-AB solution was removed by absorption on Whatman 3MM paper (Merck, Ireland) in acetonitrile washes, being the clean labelled glycans eluted in water [45].
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