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EHT Generation

EQ Eva Querdel
MR Marina Reinsch
LC Liesa Castro
DK Deniz Köse
AB Andrea Bähr
SR Svenja Reich
BG Birgit Geertz
BU Bärbel Ulmer
MS Mirja Schulze
ML Marc D. Lemoine
TK Tobias Krause
ML Marta Lemme
JS Jascha Sani
AS Aya Shibamiya
TS Tim Stüdemann
MK Maria Köhne
CB Constantin von Bibra
NH Nadja Hornaschewitz
SP Simon Pecha
YN Yusuf Nejahsie
IM Ingra Mannhardt
TC Torsten Christ
HR Hermann Reichenspurner
AH Arne Hansen
NK Nikolai Klymiuk
MK M. Krane
CK C. Kupatt
TE Thomas Eschenhagen
FW Florian Weinberger
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EHTs were generated from cells and fibrinogen/thrombin as previously described.9 For this study, the devices for casting and culturing EHTs were adapted to reach the geometries required for the intended use. Standard EHT patches (1.5×2.5 cm) were generated in a 6-well format (Figure I in the Data Supplement) with 9 to 15×106 hiPSC cardiomyocytes per 1.5 mL. For human-scale EHT patches (5.0×7.0 cm) 430 to 450×106 cells were resuspended in 25 mL.

Copyright and License information:  ©2021 The Authors.
Circulation is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDerivs License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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