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2.5. Determining the Limit of Blank (LoB) and Limit of Detection (LoD)
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The LoB and LoD were estimated according to the CLSI EP-17A2 guidelines [30]. For LoB, the 95th percentile of 50 samples with no cystatin-C was determined. Six samples with the lowest cystatin-C concentration were measured in triplicate for the consecutive five days to calculate the LoD. LoD was calculated as LoD = LoB +1.65 SD samples, where [31]
The glomerular filtration rate (GFR) was calculated by using the cystatin-C formula given by Stevens et al. [32]:
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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