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4.10. Generation of Recombinant Cells Expressing CA-STAT3

IC Il Je Cho
JK Jae Kwang Kim
EK Eun Ok Kim
SP Sang Mi Park
SK Sang Chan Kim
SK Sung Hwan Ki
SK Sae Kwang Ku
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Huh7 cells that had been transfected with either CA-STAT3 or pCDNA3.2/V5-DEST were subsequently maintained in a growth medium containing 500 μg/mL of G418 disulfate (Roche Molecular Biochemicals, Indianapolis, IN, USA). Isolated resistance colonies were pooled and used as recombinant cells. Ectopic expression of CA-STAT3 was verified by Flag immunoblotting.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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