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2.5.2. Lactate Dehydrogenase (LDH) Assay

MF Moto Fukai
TN Takuya Nakayabu
SO Shintaro Ohtani
KS Kengo Shibata
SS Shingo Shimada
SS Soudai Sakamoto
HF Hirotoshi Fuda
TF Takayuki Furukawa
MW Mitsugu Watanabe
SH Shu-Ping Hui
HC Hitoshi Chiba
TS Tsuyoshi Shimamura
AT Akinobu Taketomi
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Cellular injury was evaluated based on LDH activity in the test solution or culture medium using the Takara Cytotoxicity Detection Kit™ (Takara Bio Inc., Shiga, Japan), according to the manufacturer’s instructions. To know the total cell number just before cold preservation, cells were collected in phosphate buffered saline with 0.2% Tween20 (PBS-T) by scraping and vigorous pipetting (8 wells/plate). Then, collected cells were lysed by freeze and thaw, and sonication thereafter. LDH activity in this lysate served as the internal control in each plate. All values are expressed as a percentage compared to the lysate before cold preservation, which indicates the dead cell ratio (%).

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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