4.4. Cell Culture Establishment

Melanoma cells were isolated from pathologically-confirmed metastatic malignant melanomas and long-term cell cultures were established as previously described [68]. For this study, cells were revived and cultured for 72 h in Dulbecco’s modified Eagle medium (DMEM) containing 10% fetal calf serum (FCS) and labeled as 53C and 60C. Cell lines were checked for typical growth morphology, immunophenotype and in vitro differentiation potential [33,65].