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Luciferase Reporter Gene Assay

XM Xiao Ma
YL Yanyan Li
YS Yuntao Song
GX Guohui Xu
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Luciferase reporter gene assays were performed by using the Dual-luciferase Reporter Assay System (Promega, USA). Briefly, cells were treated with the miR-422a mimic, or control mimic, the vector containing CCDC26, CCDC26 mutant, EZH2, EZH2 mutant, Sirt6, and Sirt6 mutant fragment were transfected into the cells by using Lipofectamine 2000 (Invitrogen, USA), followed by the analysis of luciferase activities, in which Renilla was applied as a normalized control.31

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