2.2. Targeted sequence capture and high‐throughput sequencing

One hundred and twenty‐seven deafness genes (Table S1) were sequenced and captured in this study. The experimental procedures were referred to our previously published article (Li et al., 2016; Li et al., 2019). Briefly, probe capture panels (Roche NimbleGen Inc.) were designed for 127 deafness genes, and total size for targeted regions (exons, splicing sites, and immediate flanking intron sequences of 100 bp) was 619167 bp. DNA was fragmented, and the fragmented DNA was end‐repaired and ligated to the adapter oligonucleotides. After that, PCR was used to make a library preenrichment amplification. The qualified libraries were used for the capture of fragments of 127 genes. The capture of 127 genes was performed according to the NimbleGen capture protocol. The captured fragments were further sequenced by HiSeq 2500 Analyzers (Illumina).