2.3. Pathogenicity prediction of mutation sites and analysis of protein function impact

Applied Protein Variation Effect Analyzer (http://provean.jcvi.org/index.php), Sorting Intolerant from Tolerant (http://sift.jcvi.org), PolyPhen‐2 (http://genetics.bwh.harvard.edu/pph2), and Mutation Taster (http://Mutationtaster.org) for predictive analysis of protein and structural hazards. For the prediction of gene effect on protein structure, we use the following three‐dimensional protein structure model to forecast (http://www.proteinmodelportal.org).