4.6. Microtubule Staining

CCL-13 cells were cultured in 96-well plates at a density of 2 × 10³ cells/well, in 395 µL/well culture medium. Microtubules were stained with 50 nM SiR-tubulin/well (CY-SC002, Cytoskeleton, Inc., Denver, CO, USA). CCL-13 cells were subjected to SMG for 72 h, and the structure of microtubule bundles was evaluated under a Cytell microscope (GE Healthcare, Chicago, IL, USA).