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4.11. NADP/NADPH Assay

NM Nathan J. Martin
BC Belal Chami
AV Abigail Vallejo
AM Albaraa A. Mojadadi
PW Paul K. Witting
GA Gulfam Ahmad
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Total NADP/NADPH, and individual NADP+ and NADPH levels were quantified via commercially available luminescent assay (Promega Corporation, Madison, WI, USA). Briefly, cells were seeded into a Corning™ 96-well White Microplate at a previously optimised 15,000 cells/well and treated as above. The assay was run as per the manufacture’s guidelines following the modified protocol for separate measurement of cellular NADP+ and NADPH with luminescence read at 30 and 60 min using an Infinite® M200 PRO Plate reader (Tecan, Männedorf, Germany). Data was recorded and analysed in Microsoft® Excel® (2013, v7) and normalised against total cellular protein following BCA protein assay on the same plate.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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