4.3. Oleic Acid Treatment

To increase the number of LDs in cells, oleic acid (OA) (n-9 MUFA, C18:1)—a long-chain fatty acid—was used as a treatment on cells. OA was purchased from Sigma (Sigma-Aldrich, St. Louis, MO, United States) and dissolved in 0.1% NaOH and 10% bovine serum albumin (BSA). OA was prepared as a 10 mM stock solution and stored at −20 °C until required. BSA was used as a vehicle control. Cells were treated with 500 μM OA in DMEM (+1%BSA) for 6 h.