GST pulldown assay

WS Wengui Shi
GZ Gengyuan Zhang
ZM Zhijian Ma
LL Lianshun Li
ML Miaomiao Liu
LQ Long Qin
ZY Zeyuan Yu
LZ Lei Zhao
YL Yang Liu
XZ Xue Zhang
JQ Junjie Qin
HY Huili Ye
XJ Xiangyan Jiang
HZ Huinian Zhou
HS Hui Sun
ZJ Zuoyi Jiao
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SHCBP1, PLK1, and MISP gene were cloned into the pETDuetM-6HIS-GST-pp vector. GST-tagged SHCBP1, His-tagged PLK1, and His-tagged MISP proteins were expressed in the Escherichia coli strain BL21 (DE3) strain and GST pulldown assay was conducted, using a pierce GST protein interaction pulldown kit (Thermo Scientific). Input and eluate samples were resolved using SDS–PAGE and analyzed by Coomassie blue staining or immunoblotting.

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